ELISA 2.0 kit for measuring Canis familiaris allergen,Can f 1. | |||||||
| Antibodies: | 10D4/pAb | ||||||
| Standard Range: | 50-0.1 ng/mL | ||||||
| Limit of Detection: | 0.39ng/mL | ||||||
| Background: | OD<0.08 at 450nm | ||||||
| Coefficient of Determination: | R-squared>0.98 | ||||||
| Contents: | Plate: Pre-coated with anti-Can f 1 monoclonal antibody 10D4Vial 1: (white top) Can f 1 allergen standardConcentration: 500 ng/mlVial 2: (brown) Rabbit anti Can f 1 antibodyVial 3: (blue top) Peroxidase-conjugated Goat Anti-RabbitBottle 1: Wash buffer, (10x concentrate)Bottle 2: Assay buffer, (10x concentrate)Bottle 3: TMB developing substrateBottle 4: Stop solution (0.5N sulfuric acid) | ||||||
| Storage: | The ELISA 2.0 kit should be stored at 2-8°C. | ||||||
| Expiry: | 6 months from date of receipt | ||||||
| Not Provided: | Type I ultrapure water or 18.2MΩ de-ionized waterVolumetric measuring equipment (e.g. serological pipette, graduated cylinder)Clean containers for buffer and reagent preparationCalibrated single and multi-channel micropipettes and tipsVortex mixerPlate reader capable of reading absorbance at 450nmAnalysis software (recommended, but not required) | ||||||
| Notes: | The allergen standard is recommended for immunoassay calibrationpurposes only.A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website. | ||||||
| Product Resources: | EPC-CF1-1 Certificate of Analysis Validation Performance Data | ||||||
For research and commercial use in vitro:not for human in vivo or therapeutic use. | |||||||
| Certificate of Analysis | |||||||
| Pre-coated Plate: | 96-well polystyrene microtiter plate coated withmonoclonal antibody 10D4 and treated with stabilizingagent. Sealed in foil pouch with desiccant. | ||||||
| Monoclonal Antibody: | 10D4 (clone 10D4 C4 A10) | ||||||
| Immunogen: | Can f 1 | ||||||
| Isotype: | Mouse IgG2a/kappa | ||||||
| Specificity: | Binds to an epitope on dog Canis familiaris allergen, Can f 1. | ||||||
| Purification: | Produced in ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. | ||||||
| Certificate of Analysis | |||||||
| Detection Antibody: | Rabbit polyclonal antiserum | ||||||
| Immunogen: | Dog fur extract | ||||||
| Isotype | Multiple | ||||||
| Specificity: | The antiserum contains IgG antibodies to dogCanis familiaris allergen, Can f 1, as well as IgG antibodies to other dog allergens.. | ||||||
| Purification: | Produced in ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. | ||||||
| Activity: | Titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free. | ||||||
| Certificate of Analysis | |||||||
| Allergen Standard: | Purified natural Can f 1 prepared in 1% BSA/50% glycerol/PBS, pH 7.4. | ||||||
| Concentration/Calibration: | 500 ng/mL (based on amino acid analysis) | ||||||
| References: | |||||||
| 1. Larsen JN, Ford A, Gjesing B, et al. The collaborative study of the international standard of dog, Canis domesticus, hair/dander extract. J Allergy Clin Immunol 1988; 82:318-330. | |||||||
| 2. DeGroot H, Goei K, Van Sicter P, et al. Affinity purification of a major and minor allergen from dog extract: Serologic activity of affinity-purified Can f 1 and Can f 1-depleted extract. J Allergy Clin Immunol 1991; 87:1056-61. | |||||||
| 3. Schou C, Hansen G, Linter T, et al. Assay for the major dog allergen, Can f 1 investigation of house dust samples and commercial dog extracts. J Allergy Clin Immunol 1992; 88:847-53. | |||||||
| 4. Ingram JM, Sporik R, Rose G, Honsiger R, Chapman MD, Platts-Mills TAE. Quantitative assessment of exposure to dog (Can f 1) and cat (Fel d 1) allergens: Relationship to sensitization and asthma among children living in Los Alamos, New Mexico. J Allergy Clin Immunol 1995; 96:449-456. | |||||||
| 5. Sporik RB, Ingram JM, Price W, Sussman JH, Honsiger RW, Platts-Mills TAE. Association of asthma with serum IgE and skin test reactivity to allergens among children living at high altitude: Tickling the dragon’s breath. Amer J Resp Crit Care Med 1995;151:1388-1392. | |||||||
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