Plate: Pre-coated with anti-Fel d 4 monoclonal antibody 5F3Vial 1: (white top) Fel d 4 allergen standardConcentration: 100ng/mlVial 2: (brown) Rabbit anti Fel d 4 antibodyVial 3: (blue top) Peroxidase-conjugated Goat Anti-RabbitBottle 1: Wash buffer, (10x concentrate)Bottle 2: Assay buffer, (10x concentrate)Bottle 3: TMB developing substrateBottle 4: Stop solution (0.5N sulfuric acid)

Type I ultrapure water or 18.2MΩ de-ionized waterVolumetric measuring equipment (e.g. serological pipette, graduated cylinder)Clean containers for buffer and reagent preparationCalibrated single and multi-channel micropipettes and tipsVortex mixerPlate reader capable of reading absorbance at 450nmAnalysis software (recommended, but not required)

EPC-FD4-1 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro:not for human in vivo or therapeutic use.

Binds to an epitope on cat Felis domesticus allergen, Fel d 4.

References:

1. Platts-Mills TA, Vervloet D, Thomas WR, Aalberse RC, Chapman MD. Indoorallergens and asthma: report of the Third International Workshop. J Allergy ClinImmunol 1997; 100(6 Pt 1):S2-24.

2. Platts-Mills TA, Vaughan J, Squillace S, Woodfolk J, Sporik R. Sensitisation,asthma, and a modified Th2 response in children exposed to cat allergen: apopulation-based cross-sectional study. Lancet 2001; 357(9258):752-756.

3. Heinrich J, Bedada GB, Zock JP, Chinn S, Norback D, Oliveri M et al. Cat allergen level: its determinants and relationships to specific IgE to cat across European centers. J Allergy Clin Immunol 2006; 118(3):674-681

4. Jarvis D, Zock JP, Heinrich J, Svanes C, Verlato G, Olivieri M et al. Cat and dustmite allergen levels, specific IgG and IgG(4), and respiratory symptoms in adults. JAllergy Clin Immunol. 2007 Mar; 119(3):697-704.