ELISA 2.0 kit for measuring Mouse Urinary Protein (MUP) allergen, Mus m 1. | |||
| Antibodies: | pAb/pAb | ||
| Standard Range: | 25-0.05 ng/mL | ||
| Limit of Detection: | 0.19ng/mL | ||
| Background: | OD<0.08 at 450nm | ||
| Coefficient of Determination: | R-squared>0.98 | ||
| Contents: | Plate: Pre-coated with anti-Mus m 1 polyclonal rabbit capture antibody.Vial 1: (white top) Mus m 1 allergen standardConcentration: 250 ng/mlVial 2: (brown) Biotinylated polyclonal rabbit anti-Mus m 1 antibody.Vial 3: (blue top) Streptavidin-peroxidaseBottle 1: Wash buffer, (10x concentrate)Bottle 2: Assay buffer, (10x concentrate)Bottle 3: TMB developing substrateBottle 4: Stop solution (0.5N sulfuric acid) | ||
| Storage: | Store at 2-8°C | ||
| Expiry: | 6 months from date of receipt | ||
| Not Provided: | Type I ultrapure water or 18.2MΩ de-ionized waterVolumetric measuring equipment (e.g. serological pipette, graduated cylinder)Clean containers for buffer and reagent preparationCalibrated single and multi-channel micropipettes and tipsVortex mixerPlate reader capable of reading absorbance at 450nmAnalysis software (recommended, but not required) | ||
| Notes: | The allergen standard is recommended for immunoassay calibration purposes only.A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website. | ||
| Product Resources: | EPC-MM1-1 Certificate of Analysis Validation Performance Data | ||
For research and commercial use in vitro:not for human in vivo or therapeutic use. | |||
| Certificate of Analysis | |||
| Pre-coated Plate: | 96-well polystyrene microtiter plate coated withRabbit polyclonal antiserum and treated with stabilizingagent. Sealed in foil pouch with desiccant. | ||
| Coating Antibody: | Rabbit polyclonal antiserum | ||
| Immunogen: | Mus m 1 | ||
| Isotype: | Multiple | ||
| Specificity: | Binds to an epitope on mouse Mus musculus urinary protein allergen, Mus m 1. | ||
| Purification: | Affinity chromatography using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE. | ||
| Certificate of Analysis | |||
| Detection Antibody: | Rabbit polyclonal antiserum | ||
| Immunogen: | Mus m 1 | ||
| Isotype: | Multiple | ||
| Specificity: | Binds to an epitope on mouse Mus musculus urinary protein allergen, Mus m 1. | ||
| Purification: | Affinity chromatography using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE. | ||
| Biotinylation: | Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free. | ||
| Certificate of Analysis | |||
| Allergen Standard: | Purified natural Mus m 1 prepared in 1% BSA/50%glycerol/PBS, pH 7.4. | ||
| Concentration: | 250 ng/mL (based on amino acid analysis) | ||
References: 1.Phipatanakul W, Eggleston PA, Wright EC, Wood RA. Mouse allergen. I. The prevalence of mouse allergen in inner-city homes. The National Cooperative Inner-City Asthma Study. J Allergy Clin Immunol. 2000;106:1070-4. 2.Phipatanakul W, Eggleston PA, Wright EC, Wood RA. Mouse allergen. II. The relationship of mouse allergen exposure to mouse sensitization and asthma morbidity in inner-city children with asthma. J Allergy Clin Immunol. 2000;106:1075-80. 3.Permaul P, Hoffman E, Fu C, Sheehan W, Baxi S, Gaffin J, Lane J, Bailey A, King E, Chapman M, Gold D, Phipatanakul W. Allergens in urban schools and homes of children with asthma. Pediatr Allergy Immunol. 2012;23:543-9. 4. Ahluwalia SK, Peng RD, Breysse PN, Diette GB, Curtin-Brosnan J, Aloe C, Matsui EC. Mouse allergen is the major allergen of public health relevance in Baltimore City. J Allergy Clin Immunol. 2013;132:830-5. 5. Ownby DR. Will the real inner-city allergen please stand up? J Allergy Clin Immunol. 2013;132:836-7. | |||
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