ELISA 2.0 kit for measuring Rat Urinary Protein (RUP) allergen,Rat n 1.

Plate: Pre-coated with anti-Rat n 1 monoclonal antibody RUP-6Vial 1: (white top) Rat n 1 allergen standardConcentration: 500 ng/mlVial 2: (brown) Biotinylated monoclonal antibody RUP-1Vial 3: (blue top) Streptavidin-peroxidaseBottle 1: Wash buffer, (10x concentrate)Bottle 2: Assay buffer, (10x concentrate)Bottle 3: TMB developing substrateBottle 4: Stop solution (0.5N sulfuric acid)

Type I ultrapure water or 18.2MΩ de-ionized waterVolumetric measuring equipment (e.g. serological pipette, graduated cylinder)Clean containers for buffer and reagent preparationCalibrated single and multi-channel micropipettes and tipsVortex mixerPlate reader capable of reading absorbance at 450nmAnalysis software (recommended, but not required)

EPC-RN1-5 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro:not for human in vivo or therapeutic use.

Binds to an epitope on rat Rattus norvegicus urinary allergen, Rat n 1.

References:

1. Renstrom A, Larsson PH, Malmberg P, Bayard C. A new amplified monoclonal rat allergen assay used for evaluation of ventilation improvements in animal rooms. J Allergy Clin Immunol. 1997 Nov;100(5):649-55.

2. Renstrom A, Gordon S, Larsson PH, Tee RD, Newman Taylor AJ, Malmberg P. Comparison of a radioallergosorbent (RAST) inhibition method and a monoclonal enzyme linked immunosorbent assay (ELISA) for aeroallergen measurement. Clin Exp Allergy. 1997 Nov;27(11):1314-21.