ELISA 2.0 kit for measuring Oryctolagus cuniculus allergen, Ory c 3. | |||
| Antibodies: | pAb/pAb | ||
| Standard Range: | 20-0.04 ng/mL | ||
| Limit of Detection: | 0.08ng/mL | ||
| Background: | OD<0.08 at 450nm | ||
| Coefficient of Determination: | R-squared>0.98 | ||
| Contents: | Plate: Pre-coated with anti-Ory c 3 purified polyclonal rabbit antiserum.Vial 1: (white top) Ory c 3 allergen standardConcentration: 200ng/mlVial 2: (brown) Biotinylated purified polyclonal rabbit anti-Ory c 3 antibody.Vial 3: (blue top) Streptavidin-peroxidaseBottle 1: Wash buffer, (10x concentrate)Bottle 2: Assay buffer, (10x concentrate)Bottle 3: TMB developing substrateBottle 4: Stop solution (0.5N sulfuric acid) | ||
| Storage: | Store at 2-8°C | ||
| Expiry: | 6 months from date of receipt | ||
| Not Provided: | Type I ultrapure water or 18.2MΩ de-ionized waterVolumetric measuring equipment (e.g. serological pipette, graduated cylinder)Clean containers for buffer and reagent preparationCalibrated single and multi-channel micropipettes and tipsVortex mixerPlate reader capable of reading absorbance at 450nmAnalysis software (recommended, but not required) | ||
| Notes: | The allergen standard is recommended for immunoassay calibration purposes only.A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website. | ||
| Product Resources: | EPC-OC3-5 Certificate of Analysis Validation Performance Data | ||
For research and commercial use in vitro:not for human in vivo or therapeutic use. | |||
| Certificate of Analysis | |||
| Pre-coated Plate: | 96-well polystyrene microtiter plate coated withRabbit polyclonal antiserum and treated with stabilizingagent. Sealed in foil pouch with desiccant. | ||
| Coating Antibody: | Rabbit polyclonal antiserum | ||
| Immunogen: | Ory c 3 | ||
| Isotype: | Multiple | ||
| Specificity: | Binds to an epitope on rabbit Oryctolagus cuniculus allergen, Ory c 3. | ||
| Purification: | Produced in vivo and purified using Affinity chromatography. Single heavy and light chain bands on SDS-PAGE. | ||
| Certificate of Analysis | |||
| Detection Antibody: | Rabbit polyclonal antiserum | ||
| Immunogen: | Ory c 3 | ||
| Isotype: | Multiple | ||
| Specificity: | Binds to an epitope on rabbitOryctolagus cuniculus allergen, Ory c 3. | ||
| Purification: | Produced in vivo and purified using Affinity chromatography. Single heavy and light chain bands on SDS-PAGE. | ||
| Biotinylation: | Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free. | ||
| Certificate of Analysis | |||
| Allergen Standard: | Recombinant Ory c 3 prepared in 1% BSA/50%glycerol/PBS, pH 7.4. | ||
| Concentration: | 200ng/mL (based on amino acid analysis) | ||
References: | |||
Indoor Biotechnologies 由三家附属公司组成，INDOOR Biotechnologies Inc.（IBI），位于美国弗吉尼亚州夏洛茨维尔，INDOOR Biotechnologies Limited（IBL）位于英国卡迪夫，INDOOR Biotechnologies India（IBIndia），新设立于班加罗尔。Indoor Biotechnologies 遍布全球，在日本，中国和韩国拥有授权分销商，在全球约50个国家/地区销售。 Indoor Biotechnologies 生产纯化的天然和重组过敏原，用于研究和开发过敏诊断和疫苗。该产品系列目前包括 50 种纯化的过敏原，其制造具有高纯度和生物活性。这包括具有低内毒素含量的 LoTox™ 过敏原，专为过敏和哮喘的免疫学研究而设计。
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